Fluid flow shear stress upregulates prostanoid receptor EP2 but not EP4 in murine podocytes Journal Article


Authors: Srivastava, T; McCarthy ET; Sharma, R; Kats A; Carlton CG; Alon US; Cudmore PA; El-Meanawy A; Sharma M
Article Title: Fluid flow shear stress upregulates prostanoid receptor EP2 but not EP4 in murine podocytes
Abstract: Podocytes in the glomerular filtration barrier regulate the passage of plasma proteins into urine. Capillary pressure and ultrafiltration impact the structure and function of podocytes. The mechanism of podocyte injury by fluid flow shear stress (FFSS) from hyperfiltration in chronic kidney disease (CKD) is not completely understood. Recently, we demonstrated increased synthesis of prostaglandin E2 in podocytes exposed to FFSS. Here, we determine the effect of FFSS on prostanoid receptors EP1-EP4 in cultured podocytes and in Os/+ mouse kidney, a model of hyperfiltration. Results of RT-PCR, qRT-PCR, immunoblotting and immunofluorescence studies indicate that cultured podocytes express EP1, EP2 and EP4 but not EP3. FFSS resulted in upregulated expression of only EP2 in podocytes. Kidney immunostaining showed significantly increased expression of EP2 in Os/+ mice compared with littermate controls. These novel results suggest that EP2 may be responsible for mediating podocyte injury from hyperfiltration-induced augmented FFSS in CKD.
Journal Title: Prostaglandins & other lipid mediators
Volume: 104-105
ISSN: 1098-8823
Publisher: Elsevier Science  
Date Published: 2013-07
Start Page: 49
End Page: 57
Identifier: 10.1016/j.prostaglandins.2012.11.001.
KUMC Authors
  1. Madhulika Sharma
    15 Sharma
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