Refining membrane penetration by a combination of steady-state and time-resolved depth-dependent fluorescence quenching Journal Article


Authors: Kyrychenko, Alexander; Ladokhin, Alexey S
Article Title: Refining membrane penetration by a combination of steady-state and time-resolved depth-dependent fluorescence quenching
Abstract: Accurate determination of the depth of membrane penetration of a fluorescent probe, attached to a lipid, protein, or other macromolecule of interest, using depth-dependent quenching methodology is complicated by thermal motion in the lipid bilayer. Here, we suggest that a combination of steady-state and time-resolved measurements can be used to generate a static quenching profile that reduces the contribution from transverse diffusion occurring during the excited-state lifetime. This procedure results in narrower quenching profiles, compared with those obtained by traditional measurements, and thus improves precision in determination of the underlying depth distribution of the probe.
Journal Title: Analytical biochemistry
Volume: 446
ISSN: 1096-0309
Publisher: Academic Press  
Date Published: 2014
Start Page: 19
End Page: 21
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