The human parvovirus B19 non-structural protein 1 N-terminal domain specifically binds to the origin of replication in the viral DNA Journal Article


Authors: Tewary, Sunil Kumar; Zhao, Haiyan; Deng, Xuefeng; Qiu, Jianming; Tang, Liang
Article Title: The human parvovirus B19 non-structural protein 1 N-terminal domain specifically binds to the origin of replication in the viral DNA
Abstract: The non-structural protein 1 (NS1) of human parvovirus B19 plays a critical role in viral DNA replication. Previous studies identified the origin of replication in the viral DNA, which contains four DNA elements, namely NSBE1 to NSBE4, that are required for optimal viral replication (Guan et al., 2009). Here we have demonstrated in vitro that the NS1 N-terminal domain (NS1N) binds to the origin of replication in a sequence-specific, length-dependent manner that requires NSBE1 and NSBE2, while NSBE3 and NSBE4 are dispensable. Mutagenesis analysis has identified nucleotides in NSBE1 and NSBE2 that are critical for NS1N binding. These results suggest that NS1 binds to the NSBE1-NSBE2 region in the origin of replication, while NSBE3 and NSBE4 may provide binding sites for potential cellular factors. Such a specialized nucleoprotein complex may enable NS1 to nick the terminal resolution site and separate DNA strands during replication.
Keywords: Humans; Virus Replication; Protein Structure, Tertiary; Cell Line; Binding Sites; Protein Binding; DNA Replication; Gene Expression Regulation, Viral; DNA, Viral; Parvoviridae Infections; Parvovirus B19, Human; Viral Nonstructural Proteins; Replication Origin
Journal Title: Virology
Volume: 449
ISSN: 1096-0341
Publisher: Academic Press  
Date Published: 2014
Start Page: 297
End Page: 303
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