Analysis of the N-terminal positively charged residues of the simian immunodeficiency virus Vif reveals a critical amino acid required for the antagonism of rhesus APOBEC3D, G, and H Journal Article


Authors: Schmitt, Kimberly; Katuwal, Miki; Wang, Yaqiong; Li, Cicy; Stephens, Edward B
Article Title: Analysis of the N-terminal positively charged residues of the simian immunodeficiency virus Vif reveals a critical amino acid required for the antagonism of rhesus APOBEC3D, G, and H
Abstract: Previous studies have shown that apolipoprotein B mRNA editing, enzyme catalytic, polypeptide G (APOBEC3G; hA3G) and F (APOBEC3F; hA3F) proteins interact with a nonlinear binding site located at the N-terminal region of the HIV-1 Vif protein. We have analyzed the role of 12 positively charged amino acids of the N-terminal region of the SIV Vif. Simian-human immunodeficiency viruses (SHIV) were constructed that expressed each of these amino acid substitutions. These viruses were examined for replication in the presence of rhesus macaque APOBEC3 proteins (rhA3A-rhA3H), incorporation of the different A3 proteins into virions, and replication in rhesus macaque PBMC. Similar to other studies, we found that K27 was essential for rhA3G activity and rhA3F but was not important for restriction of SHIVΔvif by rhA3A, rhA3D or rhA3H. Our results identified the arginine at position 14 of the SIV Vif as a critical residue for virus restriction by rhA3D, rhA3G and rhA3H.
Keywords: Humans; Animals; Macaca mulatta; Amino Acid Motifs; Binding Sites; Amino Acid Sequence; Molecular Sequence Data; Protein Binding; Simian immunodeficiency virus; HIV-1; HIV Infections; Simian Acquired Immunodeficiency Syndrome; Cytidine Deaminase; Gene Products, vif
Journal Title: Virology
Volume: 449
ISSN: 1096-0341
Publisher: Academic Press  
Date Published: 2014
Start Page: 140
End Page: 149
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